Journal: FASEB journal : official publication of the Federation of American Societies for Experimental Biology
Article Title: Regulation and role of endoglin in cholesterol-induced endothelial and vascular dysfunction in vivo and in vitro .
doi: 10.1096/fj.201802245R
Figure Lengend Snippet: Figure 3. Western blot analysis of aortas from ApoE2/2/LDLR2/2 and WT mice. The expression of membrane Eng (A), eNOS (B), p-eNOS-Ser1177 (C), pSmad2/3 (D), MMP-14 (E), HO-1 (F), and pMLC Thr18/Ser19 (G) in total protein extracts from mice aortas. Top: densitometric analysis (control = 100%). Equal loading of samples was confirmed by immunodetection of GAPDH. Bottom: representative immunoblots. Data are shown as means 6 SEM, Mann-Whitney test (n = 7 mice/group). *P # 0.05, **P # 0.01.
Article Snippet: Primary and secondary antibodies used for Western blot analysis Mouse protein Source Catalog no. Dilution Secondary antibody dilution Eng Santa Cruz Biotechnology sc-19793 1:200 1:5000 eNOS Santa Cruz Biotechnology sc-654 1:200 1:2000 GAPDH MilliporeSigma G8795 1:10,000 1:20,000 HO-1 Abcam ab13243 1:2000 1:1000 MMP-14 Abcam ab51074 1:2000 1:1000 p-eNOS Ser1177 Santa Cruz Biotechnology sc-21871-R 1:500 1:2000 pMLC Thr18/Ser19 Cell Signaling Technology 3674 1:1000 1:2000 pSmad2/3 Cell Signaling Technology 88280 1:1000 1:2000 12 Vol.
Techniques: Western Blot, Expressing, Membrane, Control, Immunodetection, MANN-WHITNEY